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MAP1A

ANTIBODY INFORMATION
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039063   HPA039064   HPA066488  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   7   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  
Immunocytochemistry

    Approved  
Immunohistochemistry


Validated
  

Validated
    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line BJ shows localization to cytosol.  

Antibody dilution

     1:71  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.    

Expression

 RNA: detected in 31 tissues
Protein: detected in 16 cell types		   RNA: detected in 31 tissues
Protein: detected in 18 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:12000   1:7500    

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 331.3, 305.5   331.3, 305.5   331.3, 305.5  

Antibody dilution

 1:250   1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:6000   1:2850  

ANTIGEN INFORMATION
 
 

Antibody HPA039063

  

Antibody HPA039064

  

Antibody HPA066488

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 119   81   112  

Antigen sequence

 
VQEWQETSPTREEPAGEQKELAPAWEDTSPEQDNRYWRGREDVALEQDTY
WRELSCERKVWFPHELDGQGARPHYTEERESTFLDEGPDDEQEVPLREHA
TRSPWASDFKDFQESSPQK
  
SSFSHSTPSGNGKYLPGAITSPDEHILTPDSSFSKSPESLPGPALEDIAI
KWEDKVPGLKDRTSEQKKEPEPKDEVLQQKD
  
EGEREEEGRAEAPDKSSHSSKVPEASKSHATTEPEQTEPEQREPTPYPDE
RSFQYADIYEQMMLTGLGPACPTREPPLGAAGDWPPCLSTKEAAAGRNTS
AEKELSSPISPK
 

Matching transcripts

 MAP1A-001 - ENSP00000300231 [100%]
MAP1A-002 - ENSP00000371462 [100%]
  MAP1A-001 - ENSP00000300231 [100%]
MAP1A-002 - ENSP00000371462 [100%]
  MAP1A-001 - ENSP00000300231 [100%]
MAP1A-002 - ENSP00000371462 [100%]
 

Other gene match

      
ANTIGEN VIEW
MAP1A-001
MAP1A-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.