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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA016739 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA016738 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 37 cell types
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:75
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016738
Antibody HPA016739
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415395)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37, 35.7, 32.2, 30.7
37, 35.7, 32.2, 30.7, 29.1, 21.6, 15
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA016738
Antibody HPA016739
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
