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Immunohistochemical staining of human kidney shows strong luminal membrane and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows granular cytoplasmic positivity in cells in tubules and glomeruli.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity.
Expression
RNA: detected in 33 tissues Protein: detected in 34 cell types
RNA: detected in 33 tissues Protein: detected in 36 cell types
RNA: detected in 33 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:20
1:160
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015720
Antibody HPA029944
Antibody CAB034174
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
49.5, 49.1
49.5, 49.1, 44.1
49.5, 49.1, 44.1, 17.3
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA015720
Antibody HPA029944
Antibody CAB034174
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.