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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & vesicles.
Antibody dilution
1:111
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA015247
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows membranous positivity in glomeruli.
Expression
RNA: detected in 36 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015247
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402523)
Target mass (kDa)
44.9, 44.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA015247
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA015247
Antigen
Recombinant protein fragment
Length (aa)
103
Antigen sequence
IHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEH
NAALRTAGFPNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIP
TAP
