We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.
Antibody dilution
1:90
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA068087
Antibody CAB010485
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA068087
Antibody CAB010485
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
33.8, 22.9
33.8, 25.5, 22.9
Antibody dilution
1:200
1:500
PROTEIN ARRAY
Antibody HPA068087
Antibody CAB010485
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
