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Immunohistochemical staining of human lactating breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human lactating breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human lactating breast shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 3 tissues Protein: detected in 2 cell types
RNA: detected in 3 tissues Protein: detected in 0 cell types
RNA: detected in 3 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:1500
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA029855
Antibody HPA029856
Antibody CAB026343
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400829)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400829)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
16.2, 14
16.2
16.2, 14
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA029855
Antibody HPA029856
Antibody CAB026343
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
