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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:125
1:87
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011101
Antibody CAB018969
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows distinct membranous and moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 36 tissues Protein: detected in 71 cell types
RNA: detected in 36 tissues Protein: detected in 61 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:350
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011101
Antibody CAB018969
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412085)
Target mass (kDa)
28.2, 22.8, 19.8
28.2, 22.8, 21.8, 19.8, 14.7, 11.2, 6.7, 5.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA011101
Antibody CAB018969
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
