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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & cytosol.
Antibody dilution
1:59
1:116
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054406
Antibody HPA059696
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in a subset of cells in seminiferus ducts.
Expression
RNA: detected in 32 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054406
Antibody HPA059696
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
10.9
10.9, 8.5
Antibody dilution
1:290
1:390
PROTEIN ARRAY
Antibody HPA054406
Antibody HPA059696
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:2350
1:4650
ANTIGEN INFORMATION
Antibody HPA054406
Antibody HPA059696
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
39
52
Antigen sequence
SSSDSDTDVKSHAAGSKQHESIPGKAKKPKVKKKEKGKK
MEFDLGAALEPTSQKPGVGAGHGGDPKLSPHKVQGRSEAGAGPGPKQGHH
SS
