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Immunohistochemical staining of human skin shows distinct positivity in superficial layers.
Immunohistochemical staining of human breast shows extracellular and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows extracellular and cytoplasmic positivity in glandular cells
Expression
RNA: detected in 7 tissues Protein: detected in 23 cell types
RNA: detected in 7 tissues Protein: detected in 33 cell types
RNA: detected in 7 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:75
1:400
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014343
Antibody CAB025503
Antibody CAB026341
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402213)
Target mass (kDa)
32, 22.4
32, 22.4
32, 22.4
Antibody dilution
1:125
1:500
1:500
PROTEIN ARRAY
Antibody HPA014343
Antibody CAB025503
Antibody CAB026341
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
