ATXN2L - Antibodies - The Human Protein Atlas

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ATXN2L

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA041506

HPA043391

HPA071955

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:67

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA071955.
Antibody staining overlaps with antibody HPA043391.

Validated

Antibody staining overlaps with antibody HPA071955.
Antibody staining overlaps with antibody HPA041506.

Validated

Antibody staining overlaps with antibody HPA043391.
Antibody staining overlaps with antibody HPA041506.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:67

1:26

1:55

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Standard validation

Approved

Figure description

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 69 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:125

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

115.6, 113.4, 112.7, 112.1, 111.9, 110.3, 110.1

Loading control

Total protein image

Antibody dilution

1:149

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

115.6, 113.4, 112.7, 112.1, 111.9, 110.3, 110.1

115.6, 113.4, 112.7, 112.1, 111.9, 110.3, 110.1, 41.8

Antibody dilution

1:250

1:180

PROTEIN ARRAY

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:2200

ANTIGEN INFORMATION

Antibody HPA041506

Antibody HPA043391

Antibody HPA071955

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

APISASCPEPPIGSAVPTSSASIPVTSSVSDPGVGSISPASPKISLAPTD
VKELSTKEPGRTLEPQELARIAGKVPGLQNEQKRFQLEELRK

LKEEPKGKEKEVDGLLTSEPMGSPVSSKTESVSDKEDKPPLAPSGGTEGP
EQPPPPCPSQTGSPPVGLIKGED

SSYIPYNPQQFPGQPAMMQPMAHYPSQPVFAPMLQSNPRMLTSGSHPQAI
VSSSTPQYPSAEQPTPQALYATVHQSYPHHATQLHAHQP

Matching transcripts

ATXN2L-001 - ENSP00000341459 [100%]
ATXN2L-002 - ENSP00000378917 [100%]
ATXN2L-003 - ENSP00000338718 [100%]
ATXN2L-004 - ENSP00000372133 [100%]
ATXN2L-005 - ENSP00000315650 [100%]
ATXN2L-006 - ENSP00000454516 [100%]
ATXN2L-008 - ENSP00000457613 [100%]

Other gene match

ANTIGEN VIEW

ATXN2L-001	ATXN2L-002	ATXN2L-003	ATXN2L-004	ATXN2L-005	ATXN2L-006	ATXN2L-008	ATXN2L-011

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.