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TNIP2

ANTIBODY INFORMATION
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA049886   HPA063197   3082.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   13   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063197.		  
Validated

Antibody staining overlaps with antibody HPA049886.		    
 

Standard validation

 Supported   Uncertain    

     

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.    

Antibody dilution

 1:57   1:172    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.   Immunohistochemical staining of human stomach shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:850   1:400  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 48.7, 38.9, 36.7   48.7, 36.7   48.7, 38.9, 36.7  

Antibody dilution

 1:280   1:580   1:500  

PROTEIN ARRAY
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2250   1:6900    

ANTIGEN INFORMATION
 
 

Antibody HPA049886

  

Antibody HPA063197

  

Antibody CAB020818

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 80   78    

Antigen sequence

 
MLEQQILAYKDDFMSERADRERAQSRIQELEEKVASLLHQVSWRQDSREP
DAGRIHAGSKTAKYLAADALELMVPGGWRP
  
EDLNAKWQRYNASRDEYVRGLHAQLRGLQIPHEPELMRKEISRLNRQLEE
KINDCAEVKQELAASRTARDAALERVQM
  

											 
																				

																		

										
											
Matching transcripts

												
																					TNIP2-001 - ENSP00000321203 [100%] 
TNIP2-002 - ENSP00000427613 [100%] 
TNIP2-003 - ENSP00000426314 [94%] 

											 
																						TNIP2-001 - ENSP00000321203 [100%] 
TNIP2-002 - ENSP00000427613 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TNIP2-001
TNIP2-002
TNIP2-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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