GP2 - Antibodies - The Human Protein Atlas

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GP2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA015739

Antibody HPA016668

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA015739

HPA016668

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA015739

Antibody HPA016668

Immunohistochemistry

Western blot

Supported

Protein array

Approved

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA015739

Antibody HPA016668

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA016668 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA015739 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows moderate cytopalsmic positivity in exocrine glandular cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Expression

RNA: detected in 20 tissues
Protein: detected in 20 cell types

RNA: detected in 20 tissues
Protein: detected in 9 cell types

Retrieval

HIER pH6

Antibody dilution

1:300

1:1500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA015739

Antibody HPA016668

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

59.5, 59.1, 43.4, 43, 16

59.5, 59.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA015739

Antibody HPA016668

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA015739

Antibody HPA016668

Antigen

Recombinant protein fragment

Length (aa)

114

144

Antigen sequence

TVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQPQLDCGPREI
KVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASAC
RNILERNQTHAIYK

NPIEASSYGLDLDCGAPGTPEAHVCFDPCQNYTLLDEPFRSTENSAGSQG
CDKNMSGWYRFVGEGGVRMSETCVQVHRCQTDAPMWLNGTHPALGDGITN
HTACAHWSGNCCFWKTEVLVKACPGGYHVYRLEGTPWCNLRYCT

Matching transcripts

GP2-001 - ENSP00000304044 [100%]
GP2-002 - ENSP00000343861 [100%]
GP2-003 - ENSP00000370765 [100%]
GP2-004 - ENSP00000370767 [100%]
GP2-008 - ENSP00000460466 [97%]

GP2-001 - ENSP00000304044 [100%]
GP2-004 - ENSP00000370767 [100%]

Other gene match

ANTIGEN VIEW

GP2-001	GP2-002	GP2-003	GP2-004	GP2-008

RELEVANT PUBLICATIONS

Antibody HPA015739

Antibody HPA016668

Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples
Muraoka S et al
J Proteome Res 2012;11(8):4201-10

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.