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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Antibody dilution
1:128
1:93
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054383
Antibody HPA070349
Standard validation
Approved
Figure description
Immunohistochemical staining of human epididymis shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 22 tissues Protein: detected in 60 cell types
Retrieval
HIER pH6
Antibody dilution
1:1500
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054383
Antibody HPA070349
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
27.9
27.9
Antibody dilution
1:640
1:310
PROTEIN ARRAY
Antibody HPA054383
Antibody HPA070349
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
