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Antibody staining overlaps with antibody HPA007128.
Validated
Antibody staining overlaps with antibody HPA007082.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Antibody dilution
1:15
1:100
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA007082
Antibody HPA007128
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human gallbladder shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:15
1:15
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007082
Antibody HPA007128
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
141.9, 139, 108.2
141.9, 139, 108.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA007082
Antibody HPA007128
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
