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FABP4

ANTIBODY INFORMATION
 
 

Antibody HPA002188

  

Antibody CAB024961

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA002188   AF3150  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 3   5  

References

 4    

VALIDATION SUMMARY
 
 

Antibody HPA002188

  

Antibody CAB024961

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002188

  

Antibody CAB024961

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human soft tissue shows distinct positivity in adipocytes.   Immunohistochemical staining of human salivary gland shows distinct positivity in adipocytes.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 17 cell types		   RNA: detected in 34 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002188

  

Antibody CAB024961

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 14.7   14.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002188

  

Antibody CAB024961

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002188

  

Antibody CAB024961

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 128    

Antigen sequence

 
DAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITI
KSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKS
TTIKRKREDDKLVVECVMKGVTSTRVYE
  

											 
																				

																		

										
											
Matching transcripts

												
																					FABP4-001 - ENSP00000256104 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FABP4-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002188

												
										 
																				
											
											
											
Antibody CAB024961

												
										 
																		

																	

										
											
1.

												
																						Differential expression of lipid metabolism related genes in porcine muscle tissue leading to different intramuscular fat deposition

	Zhao SM et al

	Lipids 2009;44(11):1029-37

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Identification of new genes involved in human adipogenesis and fat storage

	Söhle J et al

	PLoS One 2012;7(2):e31193

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Profiling of atherosclerotic lesions by gene and tissue microarrays reveals PCSK6 as a novel protease in unstable carotid atherosclerosis

	Perisic L et al

	Arterioscler Thromb Vasc Biol 2013;33(10):2432-43

			
											 
																						
											 
																				

																		

										
											
4.

												
																						Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4

	Harjes U et al

	Oncogene 2016;Epub ahead of print

			Application: IHC, WB				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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by the Knut & Alice Wallenberg foundation.