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CD14

ANTIBODY INFORMATION
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001887   HPA002127   sc-58951   AMAb90897   AMAb90898  

Host species

 Rabbit   Rabbit   Mouse   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 2   2   14   14   14  

References

 5   2        

VALIDATION SUMMARY
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  
Immunocytochemistry

Approved          
Immunohistochemistry

Supported   Supported  

Validated
   Supported   Supported  
Western blot

Supported   Supported     Uncertain   Uncertain  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Standard validation

 Approved          

         

Figure description

 Immunofluorescent staining of human cell line SiHa shows localization to vesicles.          

Antibody dilution

 1:36          

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.          

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

   
 

 

      

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human appendix shows cytoplasmic positivity in immune cells.   Immunohistochemical staining of human urinary bladder shows cytoplasmic positivity in immune cells.   Immunohistochemical staining of human tonsil shows strong positivity in subsets of non-germinal center cells and germinal center cells.   Immunohistochemical staining of human spleen shows cytoplasmic positivity in subset of cells in red pulp.   Immunohistochemical staining of human colon shows cytoplasmic positivity in lymphoid cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 20 cell types		   RNA: detected in 37 tissues
Protein: detected in 14 cell types		   RNA: detected in 37 tissues
Protein: detected in 14 cell types		   RNA: detected in 37 tissues
Protein: detected in 12 cell types		   RNA: detected in 37 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:300   1:70000   1:1500   1:2000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		     Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

         

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 40.1, 23.3   40.1     40.1, 23.3, 9, 6.2   40.1, 23.3, 9, 6.2  

Antibody dilution

 1:250   1:250     1:1000   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:3000   1:3000        

ANTIGEN INFORMATION
 
 

Antibody HPA001887

  

Antibody HPA002127

  

Antibody CAB033631

  

Antibody CAB072865

  

Antibody CAB072866

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 124   142        

Antigen sequence

 
PCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRV
DADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTL
EDLKITGTMPPLPLEATGLALSSL
  
LRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSAL
NSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDG
NPFLVPGTALPHEGSMNSGVVPACARSTLSVGVSGTLVLLQG
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD14-001 - ENSP00000304236 [100%] 
CD14-003 - ENSP00000426543 [100%] 
CD14-201 - ENSP00000385519 [100%] 

											 
																						CD14-001 - ENSP00000304236 [100%] 
CD14-201 - ENSP00000385519 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD14-001
CD14-003
CD14-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001887

												
										 
																				
											
											
											
Antibody HPA002127

												
										 
																				
											
											
											
Antibody CAB033631

												
										 
																				
											
											
											
Antibody CAB072865

												
										 
																				
											
											
											
Antibody CAB072866

												
										 
																		

																	

										
											
1.

												
																						Functional contribution of elevated circulating and hepatic non-classical CD14CD16 monocytes to inflammation and human liver fibrosis

	Zimmermann HW et al

	PLoS One 2010;5(6):e11049

			
											 
																							Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas

	Wu CC et al

	Mol Cell Proteomics 2010;9(6):1100-17

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						High-density lipoprotein endocytosis in endothelial cells

	Fruhwürth S et al

	World J Biol Chem 2013;4(4):131-40

			
											 
																							Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria

	Bachmann J et al

	PLoS Pathog 2014;10(4):e1004038

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14

	Tarassishin L et al

	Glia 2014;62(6):999-1013

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Increased CTLA-4(+) T cells and an increased ratio of monocytes with loss of class II (CD14(+) HLA-DR(lo/neg)) found in aggressive pediatric sarcoma patients

	Hingorani P et al

	J Immunother Cancer 2015;3:35

			Application: IHC				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						Wingless ligand 5a is a critical regulator of placental growth and survival

	Meinhardt G et al

	Sci Rep 2016;6:28127

			Application: WB				
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.