PRKCE - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

PRKCE

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Provider

Atlas Antibodies
Sigma-Aldrich

Upstate

Product name

HPA044496

HPA054252

05-625

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Antibody dilution

1:67

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuropil and neuronal cells.

Immunohistochemical staining of human cerebral cortex shows strong cytopasmic positivity in neuropil.

Expression

RNA: detected in 36 tissues
Protein: detected in 18 cell types

RNA: detected in 36 tissues
Protein: detected in 9 cell types

Retrieval

HIER pH6

Antibody dilution

1:4000

1:30000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

83.7

83.7, 23.9

83.7, 23.9, 14.4

Antibody dilution

1:250

1:330

1:500

PROTEIN ARRAY

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:30000

1:2650

ANTIGEN INFORMATION

Antibody HPA044496

Antibody HPA054252

Antibody CAB001948

Antigen

Recombinant protein fragment

Not known

Length (aa)

Antigen sequence

PPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFG
EDLMP

PSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLM
SPGENGEVRQGQAKR

Matching transcripts

PRKCE-001 - ENSP00000306124 [100%]

PRKCE-001 - ENSP00000306124 [100%]
PRKCE-008 - ENSP00000378341 [100%]

Other gene match

ANTIGEN VIEW

PRKCE-001	PRKCE-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.