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KRT19

ANTIBODY INFORMATION
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA002465   M0888  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

    

Released in version

 2   1  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA002465

  

Antibody CAB000031

  
Immunocytochemistry

Approved    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.    

Antibody dilution

 1:68    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human breast shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human breast shows strong membranous positivity in glandular cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 35 cell types		   RNA: detected in 34 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH9  

Antibody dilution

 1:250   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.1   44.1, 20.8, 20.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA002465

  

Antibody CAB000031

  

Antigen

 Recombinant protein fragment   Native protein  

Length (aa)

 126    

Antigen sequence

 
TEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTL
AETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLE
QEIATYRSLLEGQEDHYNNLSASKVL
   

Matching transcripts

 KRT19-001 - ENSP00000355124 [100%]
   

Other gene match

    
ANTIGEN VIEW
KRT19-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.