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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal and glial cells, neuropil was distinctly stained.
Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in hippocampus.
Expression
RNA: detected in 34 tissues Protein: detected in 32 cell types
RNA: detected in 34 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:6000
1:3500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036575
Antibody HPA036576
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
23.7
23.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036575
Antibody HPA036576
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
