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Immunohistochemical staining of human placenta shows strong positivity in trophoblastic cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 4 tissues Protein: detected in 60 cell types
RNA: detected in 4 tissues Protein: detected in 8 cell types
RNA: detected in 4 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:45
1:50
1:350
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052507
Antibody HPA059045
Antibody HPA060900
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
12.4, 12.3, 12.1
12.3, 12.1
12.4, 12.3
Antibody dilution
1:190
1:150
1:140
PROTEIN ARRAY
Antibody HPA052507
Antibody HPA059045
Antibody HPA060900
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
