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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Antibody dilution
1:54
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA052507
Antibody HPA055941
Antibody HPA059045
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human placenta shows strong positivity in trophoblastic cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 1 tissues Protein: detected in 60 cell types
RNA: detected in 1 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:45
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052507
Antibody HPA055941
Antibody HPA059045
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
12.4, 12.3, 12.1
12.1
12.3, 12.1
Antibody dilution
1:190
1:270
1:150
PROTEIN ARRAY
Antibody HPA052507
Antibody HPA055941
Antibody HPA059045
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
