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AQP4

ANTIBODY INFORMATION
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014784   sc-20812   AMAb90537  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 4   2   12  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.   Immunofluorescent staining of human cell line A-431 shows localization to cell junctions.    

Antibody dilution

 1:286   1:75    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows distinct positivity in a subset of glial cells.   Immunohistochemical staining of human cerebral cortex shows distinct positivity in a subset of glial cells.   Immunohistochemical staining of human lateral ventricle shows distinct positivity in subset of glial cells.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 10 cell types		   RNA: detected in 11 tissues
Protein: detected in 10 cell types		   RNA: detected in 11 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:3000   1:600   1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  
 

Standard validation

 Supported      

     

Figure description

 labelling of astrocyte endnote.      

WESTERN BLOT
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 34.8, 32.3, 31.8   34.8, 32.3, 31.8, 19.2   34.8, 32.3, 31.8, 19.2  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA014784

  

Antibody CAB005079

  

Antibody CAB058689

  

Antigen

 Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 71      

Antigen sequence

 
CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVI
DVDRGEEKKGKDQSGEVLSSV
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					AQP4-001 - ENSP00000372654 [100%] 
AQP4-003 - ENSP00000462597 [100%] 
AQP4-005 - ENSP00000393121 [100%] 
AQP4-201 - ENSP00000484446 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AQP4-001
AQP4-003
AQP4-005
AQP4-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA014784

												
										 
																				
											
											
											
Antibody CAB005079

												
										 
																				
											
											
											
Antibody CAB058689

												
										 
																		

																	

										
											
1.

												
																						Human induced pluripotent stem cells are a novel source of neural progenitor cells (iNPCs) that migrate and integrate in the rodent spinal cord

	Sareen D et al

	J Comp Neurol 2014;522(12):2707-28

			Application: ICC-IF, IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Changes in cannabinoid receptors, aquaporin 4 and vimentin expression after traumatic brain injury in adolescent male mice. Association with edema and neurological deficit

	Lopez-Rodriguez AB et al

	PLoS One 2015;10(6):e0128782

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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