Cell atlas - ZNF217 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	ZNF217
Gene description	Zinc finger protein 217
Protein class	Predicted intracellular proteins Transcription factors
Predicted localization	Intracellular
Number of transcripts	4
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nuclear speckles.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nuclear speckles (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 1 antibody. HPA051857
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nuclear speckles.

Main location

Nuclear speckles (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA051857	A-431	Nuclear speckles
HPA051857	A-431	Mitochondria
HPA051857	U-2 OS	Nuclear speckles
HPA051857	U-251 MG	Nuclear speckles

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA051857

siRNA validation

Validated: Significant downregulation > 25% by one siRNA and > 10% by the other.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

83%

69%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA051857	siRNA 1	U-2 OS
HPA051857	siRNA 2	U-2 OS
HPA051857	scrambled	U-2 OS

GENE INFORMATION
Gene name	ZNF217 (HGNC Symbol)
Synonyms	ZABC1
Description	Zinc finger protein 217 (HGNC Symbol)
Entrez gene summary
Chromosome	20
Cytoband	q13.2
Chromosome location (bp)	53567065 - 53609907
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000171940 (version 83.38)
Entrez gene	7764
UniProt	O75362 (UniProt - Evidence at protein level)
neXtProt	NX_O75362
Antibodypedia	ZNF217 antibodies

PROTEIN BROWSER

ZNF217-001	ZNF217-002	ZNF217-004	ZNF217-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ZNF217-001 ENSP00000360526 ENST00000371471	O75362 [Direct mapping] Zinc finger protein 217 Show all	Predicted intracellular proteins Transcription factors Zinc-coordinating DNA-binding domains Protein evidence (Ezkurdia et al 2014) Show all	GO:0000118 [histone deacetylase complex] GO:0000122 [negative regulation of transcription from RNA polymerase II promoter] GO:0000978 [RNA polymerase II core promoter proximal region sequence-specific DNA binding] GO:0001078 [transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0007165 [signal transduction] GO:0022008 [neurogenesis] GO:0044212 [transcription regulatory region DNA binding] GO:0045892 [negative regulation of transcription, DNA-templated] GO:0046872 [metal ion binding] Show all	1048 aa 115.3 kDa	No	0
ZNF217-002 ENSP00000394010 ENST00000437222	Q9BZ31 [Direct mapping] Zinc finger protein 217 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		149 aa 16.1 kDa	No	0
ZNF217-004 ENSP00000399672 ENST00000431687	A2A326 [Direct mapping] Zinc finger protein 217 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0046872 [metal ion binding] Show all	95 aa 10.6 kDa	No	0
ZNF217-201 ENSP00000304308 ENST00000302342	O75362 [Direct mapping] Zinc finger protein 217 Show all	Predicted intracellular proteins Transcription factors Zinc-coordinating DNA-binding domains Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000118 [histone deacetylase complex] GO:0000122 [negative regulation of transcription from RNA polymerase II promoter] GO:0000978 [RNA polymerase II core promoter proximal region sequence-specific DNA binding] GO:0001078 [transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0007165 [signal transduction] GO:0022008 [neurogenesis] GO:0044212 [transcription regulatory region DNA binding] GO:0045892 [negative regulation of transcription, DNA-templated] GO:0046872 [metal ion binding] Show all	1048 aa 115.3 kDa	No	0