SYNPO - Antibodies - The Human Protein Atlas

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SYNPO

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

PROGEN Biotechnik

Product name

HPA034631

sc-50459

61094

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.

Antibody dilution

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong membranous positivity in glomerular cells.

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Expression

RNA: detected in 36 tissues
Protein: detected in 45 cell types

RNA: detected in 36 tissues
Protein: detected in 37 cell types

RNA: detected in 36 tissues
Protein: detected in 16 cell types

Retrieval

HIER pH6

Antibody dilution

1:4000

1:200

1:120

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Not consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

99.5, 96.4, 73.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Antigen

Recombinant protein fragment

Recombinant protein

Not known

Length (aa)

Antigen sequence

TTPTKVYSEVHFTLAKPPSVVNRTARPFGIQAPGGTSQMERSPMLERRHF
GEKAPAPQPPSLPDRSPRPQRHIMSRSPMVERRMMGQRS

Matching transcripts

SYNPO-001 - ENSP00000302139 [100%]
SYNPO-002 - ENSP00000377789 [100%]
SYNPO-003 - ENSP00000428378 [100%]
SYNPO-004 - ENSP00000429268 [100%]

Other gene match

ANTIGEN VIEW

SYNPO-001	SYNPO-002	SYNPO-003	SYNPO-004

RELEVANT PUBLICATIONS

Antibody HPA034631

Antibody CAB033265

Antibody CAB037325

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.