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Antibody staining overlaps with antibody HPA050229.
Validated
Antibody staining overlaps with antibody HPA043244.
Standard validation
Supported
Approved
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Antibody dilution
1:47
1:36
Literature conformity
The subcellular location is supported by literature.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043244
Antibody HPA050229
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic and extracellular positivity in exocrine glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043244
Antibody HPA050229
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405148)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
27.6, 21.1
27.6, 22.6, 19.2
Antibody dilution
1:125
1:80
PROTEIN ARRAY
Antibody HPA043244
Antibody HPA050229
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
