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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & mitochondria.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001056
Antibody CAB005081
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows distinct nuclear positivity in neuronal cells.
Expression
RNA: detected in 32 tissues Protein: detected in 21 cell types
RNA: detected in 32 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001056
Antibody CAB005081
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
79, 78.7, 77.6, 62.4
79, 78.7, 77.6, 62.4, 14.4
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA001056
Antibody CAB005081
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
