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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Antibody dilution
1:41
1:44
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037723
Antibody HPA037724
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic and extracellular positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 2 tissues Protein: detected in 58 cell types
RNA: detected in 2 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:65
1:18
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037723
Antibody HPA037724
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408930)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
62, 18.3
62
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037723
Antibody HPA037724
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
