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Immunohistochemical staining of human small intestine shows strong membranous and moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
Expression
RNA: detected in 2 tissues Protein: detected in 61 cell types
RNA: detected in 2 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:10
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011115
Antibody HPA017291
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424328)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424328)
Target mass (kDa)
25.3
25.3, 20.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA011115
Antibody HPA017291
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
