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Antibody staining overlaps with antibody HPA030299.
Validated
Antibody staining overlaps with antibody HPA030298.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:46
1:73
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA030298
Antibody HPA030299
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Expression
RNA: detected in 36 tissues Protein: detected in 22 cell types
RNA: detected in 36 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030298
Antibody HPA030299
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404696)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404696)
Target mass (kDa)
37.1
37.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA030298
Antibody HPA030299
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
