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SLC22A13

ANTIBODY INFORMATION
 
 

Antibody HPA035603

  

Antibody HPA035962

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035603   HPA035962  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA035603

  

Antibody HPA035962

  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035603

  

Antibody HPA035962

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong luminal membranous and extracellular positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubuli.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 2 cell types		   RNA: detected in 1 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:250  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035603

  

Antibody HPA035962

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 60.9   60.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035603

  

Antibody HPA035962

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA035603

  

Antibody HPA035962

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   80  

Antigen sequence

 
PETHGQGLKDTLQDLELGPHPRSPKSVPSEKETEAKGRTSSPGVAFVSST
Y
  
WVKNHTFNLSAAEQLVLSVPLDTAGHPEPCLMFRPPPANASLQDILSHRF
NETQPCDMGWEYPENRLPSLKNEFNLVCDR
 

Matching transcripts

 SLC22A13-001 - ENSP00000310241 [100%]
  SLC22A13-001 - ENSP00000310241 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC22A13-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA035603

  

Antibody HPA035962

  

1.

 Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
Fagerberg L et al
Mol Cell Proteomics 2014;13(2):397-406
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.