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HECTD4

ANTIBODY INFORMATION
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041062   HPA045473  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041062

  

Antibody HPA045473

  
Immunocytochemistry

Approved    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & vesicles.    

Antibody dilution

 1:50    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:225   1:400  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 484.4, 470   484.4, 470  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041062

  

Antibody HPA045473

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   103  

Antigen sequence

 
KETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTL
GYGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTV
R
  
ACLLNVPIESLRLRFALLQSLNTTLETFFLPLVELRQTPMYTHSIAALLK
EAKGLIFYDTKVTVMNRVLNATVQRTADHAAPEITLDPLEIVGGEIRASE
NSY
 

Matching transcripts

 HECTD4-001 - ENSP00000366783 [100%]
HECTD4-201 - ENSP00000449784 [91%]
  HECTD4-001 - ENSP00000366783 [100%]
HECTD4-201 - ENSP00000449784 [100%]
 

Other gene match

    
ANTIGEN VIEW
HECTD4-001
HECTD4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.