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C1QA

ANTIBODY INFORMATION
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002350   sc-25856  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 2   4  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA002350

  

Antibody CAB009823

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.    

Antibody dilution

 1:20    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows distinct positivity in plasma.   Immunohistochemical staining of human stomach shows distinct positivity in plasma.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 17 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414262)		    

Target mass (kDa)

 26, 23.4   26, 23.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002350

  

Antibody CAB009823

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 134    

Antigen sequence

 
GSPGNIKDQPRPAFSAIRRNPPMGGNVVIFDTVITNQEEPYQNHSGRFVC
TVPGYYYFTFQVLSQWEICLSIVSSSRGQVRRSLGFCDTTNKGLFQVVSG
GMVLQLQQGDQVWVEKDPKKGHIYQGSEADSVFS
  

											 
																				

																		

										
											
Matching transcripts

												
																					C1QA-001 - ENSP00000363773 [100%] 
C1QA-003 - ENSP00000385564 [100%] 
C1QA-002 - ENSP00000416841 [88%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
C1QA-001
C1QA-002
C1QA-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002350

												
										 
																				
											
											
											
Antibody CAB009823

												
										 
																		

																	

										
											
1.

												
																						Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Selectivity analysis of single binder assays used in plasma protein profiling

	Neiman M et al

	Proteomics 2013;13(23-24):3406-10

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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