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Antibody staining overlaps with antibody HPA067495.
Validated
Antibody staining overlaps with antibody HPA052033.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
Antibody dilution
1:156
1:130
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA052033
Antibody HPA067495
Antibody CAB032329
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 12 tissues Protein: detected in 12 cell types
RNA: detected in 12 tissues Protein: detected in 51 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052033
Antibody HPA067495
Antibody CAB032329
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
63.8, 59.9, 59.6
63.8, 59.9, 59.6, 25.7, 24.1, 22.9
63.8, 59.9, 59.6, 25.7, 24.1, 22.9
Antibody dilution
1:300
1:430
1:500
PROTEIN ARRAY
Antibody HPA052033
Antibody HPA067495
Antibody CAB032329
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
