Fields »
ABOUT
HELP
BLOG
PTPRM

ANTIBODY INFORMATION
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
   Chemicon
  

Product name

 HPA003891   MAB3741   MAB3740  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Serum   Serum  

Other gene match

      

Released in version

 16   5   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

  Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.    

Antibody dilution

 1:114   1:200    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity with additional membranous staining in glandular cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 33 cell types		   RNA: detected in 36 tissues
Protein: detected in 49 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:1500   1:2500  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

      

Target mass (kDa)

 165.1, 163.7, 157.1   165.1, 163.7, 157.1, 32.3, 28.8, 26.5, 23, 18.7   165.1, 163.7, 157.1, 32.3, 28.8, 26.5, 23, 18.7  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA003891

  

Antibody CAB022442

  

Antibody CAB022443

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 130      

Antigen sequence

 
STRQEMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMK
TNTLSTSVPNSYYPDETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHP
AIRVADLLQHITQMKCAEGYGFKEEYEVST
     

Matching transcripts

 PTPRM-001 - ENSP00000331418 [98%]
PTPRM-003 - ENSP00000382927 [98%]
PTPRM-012 - ENSP00000463325 [89%]
     

Other gene match

      
ANTIGEN VIEW
PTPRM-001
PTPRM-003
PTPRM-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.