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CSPG4

ANTIBODY INFORMATION
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002951   HPA042785   HPA050008   sc-53389  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

        

Released in version

 4   9   16   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  
Immunocytochemistry

    Approved    
Immunohistochemistry

Approved   Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Standard validation

     Approved    

       

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & plasma membrane.    

Antibody dilution

     1:200    

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Standard validation

 Approved   Approved     Approved  

       

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 67 cell types		   RNA: detected in 31 tissues
Protein: detected in 62 cell types		     RNA: detected in 31 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:25   1:150     1:60  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

       

Figure description

        

Target mass (kDa)

 250.5   250.5   250.5   250.5  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:3000   1:80000    

ANTIGEN INFORMATION
 
 

Antibody HPA002951

  

Antibody HPA042785

  

Antibody HPA050008

  

Antibody CAB016189

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 149   29   28    

Antigen sequence

 
ILYEHEMPPEPFWEAHDTLELQLSSPPARDVAATLAVAVSFEAACPQRPS
HLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQVTQFP
SRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHL
  
LEPLHTQNTQQEALTTAHLEATLEEAGPS
  
EVVQAPRKGNLQLQGTRLSDGQGFTQDD
  

											 
																				

																		

										
											
Matching transcripts

												
																					CSPG4-001 - ENSP00000312506 [100%] 

											 
																						CSPG4-001 - ENSP00000312506 [97%] 

											 
																						CSPG4-001 - ENSP00000312506 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CSPG4-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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