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HRAS

ANTIBODY INFORMATION
 
 

Antibody HPA049830

  

Antibody CAB002015

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA049830   05-516  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 KRAS - ENSG00000133703 [100%]
NRAS - ENSG00000213281 [85%]
    

Released in version

 12   1  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA049830

  

Antibody CAB002015

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049830

  

Antibody CAB002015

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human colon shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 33 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049830

  

Antibody CAB002015

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 21.7, 21.4, 21.3, 21.2, 18.9   21.3, 18.9  

Antibody dilution

 1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA049830

  

Antibody CAB002015

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA049830

  

Antibody CAB002015

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 34    

Antigen sequence

 
EGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDV
   

Matching transcripts

 HRAS-002 - ENSP00000309845 [94%]
HRAS-004 - ENSP00000380722 [94%]
HRAS-009 - ENSP00000380723 [94%]
HRAS-201 - ENSP00000388246 [94%]
HRAS-202 - ENSP00000407586 [94%]
   

Other gene match

 KRAS - ENSG00000133703 [100%]
NRAS - ENSG00000213281 [85%]
    
ANTIGEN VIEW
HRAS-002
HRAS-004
HRAS-009
HRAS-201
HRAS-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.