CKAP5 - Antibodies - The Human Protein Atlas

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CKAP5

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA039377

Antibody HPA040375

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039377

HPA040375

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA039377

Antibody HPA040375

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA039377

Antibody HPA040375

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & cytosol.

Antibody dilution

1:200

1:185

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA039377

Antibody HPA040375

Standard validation

Supported

Figure description

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 78 cell types

Retrieval

HIER pH6

Antibody dilution

1:550

1:1500

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039377

Antibody HPA040375

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

225.5, 218.5, 16.9

Loading control

Total protein image

Antibody dilution

1:514

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

225.5, 218.5, 16.9

225.5, 218.5, 21.7, 18.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA039377

Antibody HPA040375

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

1:12000

ANTIGEN INFORMATION

Antibody HPA039377

Antibody HPA040375

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

WKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLE
AALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKE

MSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHK
LDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFI

Matching transcripts

CKAP5-002 - ENSP00000432768 [100%]
CKAP5-003 - ENSP00000346566 [100%]
CKAP5-006 - ENSP00000436769 [100%]
CKAP5-201 - ENSP00000310227 [100%]

CKAP5-002 - ENSP00000432768 [100%]
CKAP5-003 - ENSP00000346566 [100%]
CKAP5-201 - ENSP00000310227 [100%]
CKAP5-009 - ENSP00000436052 [90%]
CKAP5-010 - ENSP00000431509 [90%]

Other gene match

ANTIGEN VIEW

CKAP5-002	CKAP5-003	CKAP5-006	CKAP5-009	CKAP5-010	CKAP5-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.