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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & actin filaments.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Antibody dilution
1:10
1:56
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA023804
Antibody HPA053069
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023804
Antibody HPA053069
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.1, 23.6
26.1, 21.7, 21.5, 19
Antibody dilution
1:250
1:280
PROTEIN ARRAY
Antibody HPA023804
Antibody HPA053069
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
