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LMNB2

ANTIBODY INFORMATION
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA047863   HPA062477   MAB3536  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 14   14   11  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  
Immunocytochemistry

  Supported    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.    

Antibody dilution

   1:57    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA062477 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA047863 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear and membranous positivity in neuronal cells.   Immunohistochemical staining of human kidney shows strong nuclear membranous positivity in cells in tubules.   Immunohistochemical staining of human cerebral cortex shows strong positivity in nuclei and nuclear membranes of neurons.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		   RNA: detected in 37 tissues
Protein: detected in 38 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:6000   1:1600   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69.9   69.9   69.9  

Antibody dilution

 1:1160   1:190   1:500  

PROTEIN ARRAY
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:9300   1:2300    

ANTIGEN INFORMATION
 
 

Antibody HPA047863

  

Antibody HPA062477

  

Antibody CAB022593

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 76   52    

Antigen sequence

 
DEVNKSAKKREGELTVAQGRVKDLESLFHRSEVELAAALSDKRGLESDVA
ELRAQLAKAEDGHAVAKKQLEKETLM
  
PSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEE
EE
  

											 
																				

																		

										
											
Matching transcripts

												
																					LMNB2-001 - ENSP00000327054 [100%] 

											 
																						LMNB2-001 - ENSP00000327054 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LMNB2-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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