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ANKLE2

ANTIBODY INFORMATION
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003602   HPA074838  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA003602

  

Antibody HPA074838

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Uncertain    
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & endoplasmic reticulum.  

Antibody dilution

   1:115  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 4 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:150    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 104.1   104.1  

Antibody dilution

 1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:4600  

ANTIGEN INFORMATION
 
 

Antibody HPA003602

  

Antibody HPA074838

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   96  

Antigen sequence

 
SETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGS
GDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDE
AMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSH
  
LLEQGGRLSSFYHHEAGVTALSQDPQRILKPAEGNPTDQAGFSEDRDFGY
SVGLNPPEEEAVTSKTCSVPPSDTDTYRAGATASKEPPLYYGVCPV
 

Matching transcripts

 ANKLE2-001 - ENSP00000350686 [100%]
  ANKLE2-001 - ENSP00000350686 [100%]
 

Other gene match

    
ANTIGEN VIEW
ANKLE2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.