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TALDO1

ANTIBODY INFORMATION
 
 

Antibody HPA040373

  

Antibody HPA048089

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040373   HPA048089  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA040373

  

Antibody HPA048089

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040373

  

Antibody HPA048089

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA048089.		  
Validated

Antibody staining overlaps with antibody HPA040373.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:50   1:10  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA040373

  

Antibody HPA048089

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37.5, 35.3   37.5, 35.3  

Antibody dilution

 1:250   1:100  

PROTEIN ARRAY
 
 

Antibody HPA040373

  

Antibody HPA048089

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:800  

ANTIGEN INFORMATION
 
 

Antibody HPA040373

  

Antibody HPA048089

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   75  

Antigen sequence

 
MSSSPVKRQRMESALDQLKQFTTVVADTGDFHAIDEYKPQDATTNPSLIL
AAAQMPAYQELVEEAIAYGRKLGGS
  
FVGRILDWHVANTDKKSYEPLEDPGVKSVTKIYNYYKKFSYKTIVMGASF
RNTGEIKALAGCDFLTISPKLLGEL
 

Matching transcripts

 TALDO1-001 - ENSP00000321259 [100%]
TALDO1-010 - ENSP00000437098 [100%]
  TALDO1-001 - ENSP00000321259 [100%]
TALDO1-010 - ENSP00000437098 [100%]
 

Other gene match

    
ANTIGEN VIEW
TALDO1-001
TALDO1-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.