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CHD9

ANTIBODY INFORMATION
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042053   HPA049420  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA042053

  

Antibody HPA049420

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles, plasma membrane & cytosol.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:20  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 326, 324.1, 70.8   326, 324.1  

Antibody dilution

 1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA042053

  

Antibody HPA049420

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   119  

Antigen sequence

 
HQVESQTEPFTGLDPEDLLQEGLLPHFDESTFGQDNSSHILDHDLDRQFT
SHLVTRPSDMAQTQLQSQARSWHSSFSNHQHLHDRNHLCLQRQPPSSKKS
DGSGTYTKLQNTQV
  
RNYSQSKMAHSRTSTPLLQQYQVALSASPLTSLPRLLDAKGIILEEMKVK
SENLKEEPQSSEEESMSSVETRTLIKSEPVSPKNGVLPQATGDQKSGGKC
ETDRRMVAARTEPLTPNPA
 

Matching transcripts

 CHD9-001 - ENSP00000457466 [100%]
CHD9-002 - ENSP00000456793 [100%]
CHD9-003 - ENSP00000455307 [100%]
CHD9-020 - ENSP00000381522 [100%]
CHD9-201 - ENSP00000396345 [100%]
  CHD9-001 - ENSP00000457466 [100%]
CHD9-003 - ENSP00000455307 [100%]
CHD9-020 - ENSP00000381522 [100%]
CHD9-201 - ENSP00000396345 [100%]
 

Other gene match

    
ANTIGEN VIEW
CHD9-001
CHD9-002
CHD9-003
CHD9-020
CHD9-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.