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SLC25A20

ANTIBODY INFORMATION
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016862   HPA029863  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   6  

References

 1   1  

VALIDATION SUMMARY
 
 

Antibody HPA016862

  

Antibody HPA029863

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & mitochondria.  

Antibody dilution

   1:43  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

 

  

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 52 cell types		   RNA: detected in 37 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:60   1:10  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424755)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424755)		  

Target mass (kDa)

 32.9, 25.1   32.9, 25.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA016862

  

Antibody HPA029863

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 41   131  

Antigen sequence

 
TVKVRLQTQPPSLPGQPPMYSGTFDCFRKTLFREGITGLYR
  
SGESKYTGTLDCAKKLYQEFGIRGIYKGTVLTLMRDVPASGMYFMTYEWL
KNIFTPEGKRVSELSAPRILVAGGIAGIFNWAVAIPPDVLKSRFQTAPPG
KYPNGFRDVLRELIRDEGVTSLYKGFNAVMI
 

Matching transcripts

 SLC25A20-001 - ENSP00000326305 [100%]
SLC25A20-002 - ENSP00000388986 [83%]
  SLC25A20-001 - ENSP00000326305 [100%]
SLC25A20-002 - ENSP00000388986 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC25A20-001
SLC25A20-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA016862

  

Antibody HPA029863

  

1.

 Regulation of the human SLC25A20 expression by peroxisome proliferator-activated receptor alpha in human hepatoblastoma cells
Tachibana K et al
Biochem Biophys Res Commun 2009;389(3):501-5
   Plasma profiling reveals three proteins associated to amyotrophic lateral sclerosis
Häggmark A et al
Ann Clin Transl Neurol 2014;1(8):544-53
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.