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ERBB4

ANTIBODY INFORMATION
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
   R&D Systems
  

Product name

 HPA012016   RB9045   MAB1131  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

      

Released in version

 5   1   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate nuclear and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 66 cell types		   RNA: detected in 12 tissues
Protein: detected in 58 cell types		   RNA: detected in 12 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:50   1:150   1:300  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 146.8, 145.2, 140.3   146.8, 145.2, 140.3, 80.9, 14.6   146.8, 145.2, 140.3, 80.9, 14.6  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA012016

  

Antibody CAB000276

  

Antibody CAB025522

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 103      

Antigen sequence

 
LPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRARIDSN
RNQFVYRDGGFAAEQGVSVPYRAPTSTIPEAPVAQGATAEIFDDSCCNGT
LRK
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ERBB4-002 - ENSP00000403204 [100%] 
ERBB4-001 - ENSP00000342235 [87%] 
ERBB4-201 - ENSP00000385565 [87%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ERBB4-001
ERBB4-002
ERBB4-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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