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Antibody staining overlaps with antibody HPA038482.
Validated
Antibody staining overlaps with antibody HPA038013.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.
Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol.
Antibody dilution
1:200
1:164
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038013
Antibody HPA038482
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA038482 across 71 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA038013 across 71 cell types.
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules with distinct extracellular material.
Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 5 tissues Protein: detected in 62 cell types
RNA: detected in 5 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:300
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038013
Antibody HPA038482
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56.6
56.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA038013
Antibody HPA038482
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.