PFAS - Antibodies - The Human Protein Atlas

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PFAS

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA022140

Antibody HPA022886

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022140

HPA022886

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA022140

Antibody HPA022886

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA022140

Antibody HPA022886

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:7

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA022140

Antibody HPA022886

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022886 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022140 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 46 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:20

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022140

Antibody HPA022886

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

144.7, 14.9, 14.6

144.7

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA022140

Antibody HPA022886

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA022140

Antibody HPA022886

Antigen

Recombinant protein fragment

Length (aa)

103

Antigen sequence

LFGCPLLLDDVARESWLLPGSNDLLLEVGPRLNFSTPTSTNIVSVCRATG
LGPVDRVETTRRYRLSFAHPPSAEVEAIALATL

ALERVLRLPAVASKRYLTNKVDRSVGGLVAQQQCVGPLQTPLADVAVVAL
SHEELIGAATALGEQPVKSLLDPKVAARLAVAEALTNLVFALVTDLRDVK
CSG

Matching transcripts

PFAS-001 - ENSP00000313490 [100%]
PFAS-007 - ENSP00000464689 [88%]
PFAS-201 - ENSP00000487260 [80%]

PFAS-001 - ENSP00000313490 [100%]

Other gene match

ANTIGEN VIEW

PFAS-001	PFAS-007	PFAS-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.