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EDC3

ANTIBODY INFORMATION
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040650   HPA044206   HPA066137  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 11   11   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line MCF7 shows localization to cytoplasmic bodies.  

Antibody dilution

     1:65  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subsets of cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in renal tubules.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 66 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:500   1:75    

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.1, 28, 19.2, 13.8, 13, 10.6, 9.8   56.1   56.1, 23.3, 18.5  

Antibody dilution

 1:250   1:250   1:144  

PROTEIN ARRAY
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:12000   1:2600  

ANTIGEN INFORMATION
 
 

Antibody HPA040650

  

Antibody HPA044206

  

Antibody HPA066137

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   95   85  

Antigen sequence

 
WLGSIVSINCGDSLGVYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTF
RAGDITELKILEIPGPGDNQHFGDLHQTELGPSGAGCQVGINQN
  
LDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLA
LGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPL
  
IDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEF
CTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLE
 

Matching transcripts

 EDC3-001 - ENSP00000320503 [100%]
EDC3-003 - ENSP00000401343 [100%]
EDC3-004 - ENSP00000455580 [100%]
EDC3-010 - ENSP00000455589 [100%]
EDC3-013 - ENSP00000457170 [100%]
EDC3-016 - ENSP00000455503 [100%]
EDC3-017 - ENSP00000457523 [100%]
EDC3-018 - ENSP00000455870 [100%]
EDC3-014 - ENSP00000454317 [94%]
  EDC3-001 - ENSP00000320503 [100%]
EDC3-003 - ENSP00000401343 [100%]
EDC3-004 - ENSP00000455580 [100%]
  EDC3-001 - ENSP00000320503 [100%]
EDC3-003 - ENSP00000401343 [100%]
EDC3-004 - ENSP00000455580 [100%]
EDC3-005 - ENSP00000455393 [100%]
EDC3-009 - ENSP00000456805 [100%]
 

Other gene match

      
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EDC3-001
EDC3-003
EDC3-004
EDC3-005
EDC3-009
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EDC3-016
EDC3-017
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Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.