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ANTIBODY INFORMATION
 
 

Antibody CAB016084

  

Provider

 Santa Cruz Biotechnology
  

Product name

 sc-13556  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Protein A/G  

Other gene match

  

Released in version

 4  

References

  

VALIDATION SUMMARY
 
 

Antibody CAB016084

  
Immunocytochemistry

Supported  
Immunohistochemistry


Validated
  
Western blot

Uncertain  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB016084

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:25  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB016084

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center and non-germinal center cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 44 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:100  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB016084

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 123.5, 123.4, 96.5, 59.6, 38.2, 19.7  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB016084

  

Antigen

 Not known  

Length (aa)

  

Antigen sequence

 

											 
																				

																		

										
											
Matching transcripts

												
																					
											 
																				

																		

										
											
Other gene match

												
																					
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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