PUF60 - Antibodies - The Human Protein Atlas

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PUF60

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA045733

HPA052096

HPA059714

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Antibody dilution

1:22

1:62

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Standard validation

Supported

Figure description

Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 51 cell types

RNA: detected in 37 tissues
Protein: detected in 75 cell types

Retrieval

HIER pH6

Antibody dilution

1:750

1:350

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

59.9, 58.2, 57.1, 55.7, 55.4, 54, 35.7, 34.5, 28.7, 26.9, 24.8

59.9, 58.2, 57.1, 55.7, 55.4, 54, 28.1

59.9, 58.2, 57.1, 55.7, 55.4, 54, 35.7, 34.5

Antibody dilution

1:250

1:400

1:210

PROTEIN ARRAY

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3250

1:2500

ANTIGEN INFORMATION

Antibody HPA045733

Antibody HPA052096

Antibody HPA059714

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

IKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIM
CRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKG

AATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAP
GVITGVTPARPPIPVTIPSVGVVNPILASPPTL

PIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPT
TGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGG

Matching transcripts

PUF60-001 - ENSP00000434359 [100%]
PUF60-003 - ENSP00000402953 [100%]
PUF60-005 - ENSP00000395417 [100%]
PUF60-011 - ENSP00000433403 [100%]
PUF60-025 - ENSP00000436131 [100%]
PUF60-002 - ENSP00000322036 [82%]
PUF60-004 - ENSP00000322016 [82%]
PUF60-006 - ENSP00000431960 [82%]
PUF60-012 - ENSP00000434863 [82%]
PUF60-013 - ENSP00000437309 [82%]
PUF60-024 - ENSP00000432610 [82%]

PUF60-001 - ENSP00000434359 [100%]
PUF60-002 - ENSP00000322036 [100%]
PUF60-003 - ENSP00000402953 [100%]
PUF60-004 - ENSP00000322016 [100%]
PUF60-005 - ENSP00000395417 [100%]
PUF60-006 - ENSP00000431960 [100%]
PUF60-023 - ENSP00000432091 [92%]

Other gene match

ANTIGEN VIEW

PUF60-001	PUF60-002	PUF60-003	PUF60-004	PUF60-005	PUF60-006	PUF60-011	PUF60-012	PUF60-013	PUF60-023	PUF60-024	PUF60-025

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.