Fields »
ABOUT
HELP
BLOG
SCRIB

ANTIBODY INFORMATION
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Upstate
  

Product name

 HPA023557   HPA064312   sc-55543   07-643  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 11   14   11   11  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  
Immunocytochemistry


Validated

Validated
  

Validated
      
Immunohistochemistry

Supported    

Validated
   Supported  
Western blot

Uncertain   Uncertain   Supported   Supported  
Protein array

Approved   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		        

 

Relative fluorescence intensity (RFI)

        

Figure description

 Cytoplasmic region of segmented cells in 10x-images        

Antibody dilution

 1:200        
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA064312.		  
Validated

Antibody staining overlaps with antibody HPA023557.		      
 

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cell junctions.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cell junctions.      

Antibody dilution

 1:200   1:200      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported     Supported   Supported  

       

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells found in seminiferus ducts.     Immunohistochemical staining of human rectum shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		     RNA: detected in 37 tissues
Protein: detected in 36 cell types		   RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:1000     1:25   1:350  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

     Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 177.7, 174.9, 165.3   177.7, 174.9, 165.3, 68.3   177.7, 174.9, 165.3, 68.3, 14.9   177.7, 174.9, 165.3, 68.3, 14.9  

Antibody dilution

 1:250   1:1080   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:13000      

ANTIGEN INFORMATION
 
 

Antibody HPA023557

  

Antibody HPA064312

  

Antibody CAB015463

  

Antibody CAB022081

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 88   75      

Antigen sequence

 
VSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEGRRSE
ACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVS
  
RLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAALEVSPG
VIANPFAAGIGHRNSLESISSIDRE
     

Matching transcripts

 SCRIB-001 - ENSP00000322938 [100%]
SCRIB-002 - ENSP00000349486 [100%]
SCRIB-003 - ENSP00000366756 [100%]
  SCRIB-001 - ENSP00000322938 [100%]
SCRIB-002 - ENSP00000349486 [100%]
SCRIB-003 - ENSP00000366756 [100%]
SCRIB-004 - ENSP00000431519 [100%]
     

Other gene match

        
ANTIGEN VIEW
SCRIB-001
SCRIB-002
SCRIB-003
SCRIB-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.